August 20, 2020

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

Background: polymerase chain reaction (PCR) which is used to detect viral pathogens because of the high sensitivity and specificity. However, the conventional PCR method can not determine the virus infectivity. Virus infectivity is conventionally checked by methods such as plaque test, although the test like this takes a few days. Long-Range quantitative reverse-transcription PCR (RT-qPCR) has previously been suggested for a rapid assessment of RNA […]

August 20, 2020

Apigenin suppresses influenza A virus-induced RIG-I activation and viral replication

Flavonoid apigenin is low toxicity and few favorable bioactivities, including anti-tumor properties, antioxidant, anti-inflammatory activity and antiviral. However, the effect of Apigenin on influenza virus infection remains poorly understood. Thus, the purpose of this study was to determine the effect of Apigenin on influenza A virus (IAV) induced inflammation and viral replication. This study showed that treatment significantly suppressed Apigenin IAV-induced upregulation of RIG-I expression, […]

August 20, 2020

Impaired phagocytic function in CX3CR1 + tissue-resident skeletal muscle macrophages prevents muscle recovery after influenza A virus-induced pneumonia in old mice

the victim’s skeletal muscle dysfunction pneumonia disproportionately affects older individuals among major cause considerable morbidity. We found that the recovery of the skeletal muscle is impaired in elderly compared with young mice after the influenza A virus-induced pneumonia. In young mice, the recovery of muscle loss associated with the expansion of skeletal muscle tissue macrophage-resident and downregulation of the expression of MHC II, followed by […]