September 25, 2020

RNA SNHG15 regulates cardiomyocyte apoptosis after hypoxia/reperfusion damage

Integrative evaluation of lengthy non-coding RNA and mRNA in broilers with valgus-varus deformity   Background: Bone abnormality and leg illness in business broiler flocks are more and more outstanding, inflicting critical financial losses to the broiler breeding business. Valgus-varus deformity (VVD) is a standard deformity of the lengthy bone in broilers that manifests as an outward or inward deviation of the tibiotarsus or tarsometatarsus. There’s a paucity of research […]

August 20, 2020

Regulation of Host Immune Responses against Influenza A Virus Infection by Mitogen-Activated Protein Kinases (MAPKs)

Influenza is a major respiratory viral illness caused by infection of influenza A virus (IAV), which still persists in various global seasonal epidemics every year. the host immune response is a key factor that determines the severity of influenza infection, present an attractive target for the development of new therapies for the treatment. Among the double track signal transduction that regulates the activation of the […]

August 20, 2020

Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode

influenza virus have been successfully deployed using a variety of animal cell lines in batch, fed-batch and perfusion culture. For suspension cells, most studies reported on membrane-based cell retention device usually causes the accumulation of the virus in a bioreactor in perfusion mode. Virus harvest continuously aims to improve productivity, a settler tend evaluated for influenza A virus (IAV) production using the birds AGE1.CR.pIX suspension […]

August 20, 2020

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

Background: polymerase chain reaction (PCR) which is used to detect viral pathogens because of the high sensitivity and specificity. However, the conventional PCR method can not determine the virus infectivity. Virus infectivity is conventionally checked by methods such as plaque test, although the test like this takes a few days. Long-Range quantitative reverse-transcription PCR (RT-qPCR) has previously been suggested for a rapid assessment of RNA […]

August 20, 2020

Apigenin suppresses influenza A virus-induced RIG-I activation and viral replication

Flavonoid apigenin is low toxicity and few favorable bioactivities, including anti-tumor properties, antioxidant, anti-inflammatory activity and antiviral. However, the effect of Apigenin on influenza virus infection remains poorly understood. Thus, the purpose of this study was to determine the effect of Apigenin on influenza A virus (IAV) induced inflammation and viral replication. This study showed that treatment significantly suppressed Apigenin IAV-induced upregulation of RIG-I expression, […]

August 20, 2020

Impaired phagocytic function in CX3CR1 + tissue-resident skeletal muscle macrophages prevents muscle recovery after influenza A virus-induced pneumonia in old mice

the victim’s skeletal muscle dysfunction pneumonia disproportionately affects older individuals among major cause considerable morbidity. We found that the recovery of the skeletal muscle is impaired in elderly compared with young mice after the influenza A virus-induced pneumonia. In young mice, the recovery of muscle loss associated with the expansion of skeletal muscle tissue macrophage-resident and downregulation of the expression of MHC II, followed by […]