influenza virus have been successfully deployed using a variety of animal cell lines in batch, fed-batch and perfusion culture. For suspension cells, most studies reported on membrane-based cell retention device usually causes the accumulation of the virus in a bioreactor in perfusion mode.
Virus harvest continuously aims to improve productivity, a settler tend evaluated for influenza A virus (IAV) production using the birds AGE1.CR.pIX suspension cell lines. settlers tend to provide a lot of advantages because they are scalable, robust, and comply with cGMP regulations, for example, for the manufacture of recombinant proteins. perfusion levels up to 3000 L / day has been reported. In our study, the successful growth of cells AGE1.CR.pIX to 50 × 106 cells / mL and a cell retention efficiency exceeding 96% was obtained with the settlers cooled to room temperature.
No virus observed retention. A total of 5.4 to 6.5 × 1013 virions produced while control experiments with ATF system equaled 1.9 × 1013 virions. For infections of the 25 × 106 cells / mL, virus-specific cells produces up to 3474 virions / cell was obtained, approximately 5-fold higher than for cultivation based ATF conducted as controls (723 virions / cell). Trypsin activity proved to have a major impact on the dynamics of cell growth after a subsequent infection cell retention device, especially on a cell concentration of 50 × 106 cells / mL.
Further control experiments carried out by settlers acoustic show that virus production increased with the heat exchanger of the settlers tend to be operated at 27 ° C. In short, cell culture-based virus production in perfusion mode with sloping settlers and continuous harvest can drastically improve results and may IAV results of other viruses. To our knowledge, this is the first report that shows the potential of this device for the production of viral vaccines.
Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode
avoidance mechanisms of type I interferon response by influenza A virus
Type I interferon (IFN) represent the first line of host defense against influenza virus infection, and precise control of type I IFN response is the central event of immune defense against influenza virus infection. Influenza virus is one of the main causes of respiratory tract infections in humans and are responsible for seasonal epidemics and pandemics occasionally, leading to a serious threat to global human health due to antigenic variation and interspecies transmission of them.
Although the host cells have evolved mechanisms of antiviral advanced by sensing products of influenza virus and trigger signaling cascades resulting in the secretion of IFN type I (IFN-α / β), influenza viruses have developed strategies to counter these mechanisms and avoid the type I IFNs in response, for example, with induces host shut-off, or by arranging polyubiquitination viral proteins and host. This review will summarize the current knowledge about how the host cells recognize influenza virus to induce type I IFN response and the strategies used to prevent influenza virus type I IFN signaling pathways, which will help to antiviral and vaccine development.
To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
Cortisone (C21H28O5, Kendall's Compound ?E') was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cor
Description: Sandwich CLIA kit used for quantitative measuring the cortisone present in Dried Fecal Samples, Serum, Saliva, Urine, Plasma, Tissue Culture Media samples from all species
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Dehydroepiandrosterone (DHEA) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Chemiluminescent immunoassay for detection of General Dehydroepiandrosterone (DHEA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Dehydroepiandrosterone (DHEA) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Chemiluminescent immunoassay for detection of General Dehydroepiandrosterone (DHEA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Dehydroepiandrosterone (DHEA) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Chemiluminescent immunoassay for detection of General Dehydroepiandrosterone (DHEA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Dehydroepiandrosterone (DHEA) were tested on 3 different plates, 8 replicates in each plate
Description: This is Competitive Chemiluminescent immunoassay for detection of General Dehydroepiandrosterone (DHEA) in serum, plasma and other biological fluids.
Known also as Dehydroepiandrosterone Clia kit. Alternative names of the recognized antigen: n/a
Description: Competitive Inhibition chemiluminescent immunoassay for detection of General Dehydroepiandrosterone (DHEA)serum, plasma and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Human Insulin (INS) in serum, plasma, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Human Insulin (INS) in serum, plasma, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Human Insulin (INS) in serum, plasma, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Human Insulin (INS) in serum, plasma, cell culture supernates and other biological fluids.
Known also as Insulin Clia kit. Alternative names of the recognized antigen: n/a
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Human Insulin (INS)Serum, plasma, cell culture supernates and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Rat Insulin (INS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Rat Insulin (INS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Rat Insulin (INS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Insulin (INS) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of Rat Insulin (INS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Insulin Clia kit. Alternative names of the recognized antigen: n/a
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Rat Insulin (INS)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Thyroxine (T4) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Thyroxine (T4) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Thyroxine (T4) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Thyroxine (T4) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Triiodothyronine (T3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Triiodothyronine (T3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Triiodothyronine (T3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Triiodothyronine (T3) in serum, plasma and other biological fluids.
Known also as Triiodothyronine Clia kit. Alternative names of the recognized antigen: 3, 3', 5-Triiodo-L-Thyronine
Liothyronine
Cytomel
Tertroxin
Description: Competitive Inhibition chemiluminescent immunoassay for detection of General Triiodothyronine (T3)Serum, plasma and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estriol (E3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estriol (E3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estriol (E3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estriol (E3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estriol (E3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estriol (E3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estriol (E3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estriol (E3) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Testosterone (Testo) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Testosterone (Testo) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Testosterone (Testo) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Testosterone (Testo) in serum, plasma and other biological fluids.
Known also as Testosterone Clia kit. Alternative names of the recognized antigen: T
Testesteron
Testeronun
Description: Competitive Inhibition chemiluminescent immunoassay for detection of General Testosterone (Testo)serum, plasma and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Progesterone (PG) in serum, plasma and other biological fluids.
In the last months of 2019, an outbreak of fatal respiratory disease that began in Wuhan, China, and quickly spread to other parts of the world. It named COVID-19, and to date, thousands of cases of infection and deaths reported worldwide. The disease is associated with a variety of symptoms that make it difficult to diagnose accurately.
Previous SARS pandemic in 2003, researchers found that patients with fever, cough, or sore throat has influenza virus-positive rate of 5%. These findings triggered in our minds that the various symptoms and also the relatively high prevalence of death in our patients may be due to co-infection with other viruses.
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