Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode

influenza virus have been successfully deployed using a variety of animal cell lines in batch, fed-batch and perfusion culture. For suspension cells, most studies reported on membrane-based cell retention device usually causes the accumulation of the virus in a bioreactor in perfusion mode.

Virus harvest continuously aims to improve productivity, a settler tend evaluated for influenza A virus (IAV) production using the birds AGE1.CR.pIX suspension cell lines. settlers tend to provide a lot of advantages because they are scalable, robust, and comply with cGMP regulations, for example, for the manufacture of recombinant proteins. perfusion levels up to 3000 L / day has been reported. In our study, the successful growth of cells AGE1.CR.pIX to 50 × 106 cells / mL and a cell retention efficiency exceeding 96% was obtained with the settlers cooled to room temperature.

No virus observed retention. A total of 5.4 to 6.5 × 1013 virions produced while control experiments with ATF system equaled 1.9 × 1013 virions. For infections of the 25 × 106 cells / mL, virus-specific cells produces up to 3474 virions / cell was obtained, approximately 5-fold higher than for cultivation based ATF conducted as controls (723 virions / cell). Trypsin activity proved to have a major impact on the dynamics of cell growth after a subsequent infection cell retention device, especially on a cell concentration of 50 × 106 cells / mL.

Further control experiments carried out by settlers acoustic show that virus production increased with the heat exchanger of the settlers tend to be operated at 27 ° C. In short, cell culture-based virus production in perfusion mode with sloping settlers and continuous harvest can drastically improve results and may IAV results of other viruses. To our knowledge, this is the first report that shows the potential of this device for the production of viral vaccines.

Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode
Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode

avoidance mechanisms of type I interferon response by influenza A virus

Type I interferon (IFN) represent the first line of host defense against influenza virus infection, and precise control of type I IFN response is the central event of immune defense against influenza virus infection. Influenza virus is one of the main causes of respiratory tract infections in humans and are responsible for seasonal epidemics and pandemics occasionally, leading to a serious threat to global human health due to antigenic variation and interspecies transmission of them.

Although the host cells have evolved mechanisms of antiviral advanced by sensing products of influenza virus and trigger signaling cascades resulting in the secretion of IFN type I (IFN-α / β), influenza viruses have developed strategies to counter these mechanisms and avoid the type I IFNs in response, for example, with induces host shut-off, or by arranging polyubiquitination viral proteins and host. This review will summarize the current knowledge about how the host cells recognize influenza virus to induce type I IFN response and the strategies used to prevent influenza virus type I IFN signaling pathways, which will help to antiviral and vaccine development.

Basic Cytotoxicity Test Kits

CT17002 250 Tests
EUR 650

Total Cytotoxicity Test Kits

CT17003 125 Tests
EUR 513

Total Cytotoxicity Test Kits

CT17004 250 Tests
EUR 813

Pipette Stand Starter Kits

P7700-S1 1 PC
EUR 507.83

Pipette Stand Starter Kits

P7700-S2 1 PC
EUR 526.1

Rat Neural Stem Cell Kits

PC37123 1 X 10(6) *minimum order 6 vials*
EUR 957

MitoPT™ TMRE & TMRM Kits

KF17358 500 Test
EUR 479

MitoPT™ TMRE & TMRM Kits

KF17359 500 Test
EUR 479

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 565
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1335
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2019
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

OKSA11298 500 Tests
EUR 956
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

OKSA11300 500 Tests
EUR 956
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 221
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 340
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-MTT Cell Viability Assay Kits

CQMT-500 500
EUR 210
Description: Colorimetric (570nm) assay for cell viability, proliferation, cytotoxcity, HTS for anticancer agents. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

Nori® Porcine 3-Plex ELISA Kits-Interferons

GR224009 96-well
EUR 503

Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins

GR224017 96-well
EUR 545

Nori® Porcine 6-Plex ELISA Kits-BMP

GR224025 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-CCL

GR224026 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-MMP

GR224027 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-TLR

GR224028 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-Hormones

GR224029 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-SAA

GR224030 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-S100

GR224031 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-CXCL

GR224032 96-well
EUR 629

Nori® Equine 3-Plex ELISA Kits-Interferons

GR225009 96-well
EUR 503

Nori® Equine 4-Plex ELISA Kits-Immunoglobulins

GR225017 96-well
EUR 545

Nori® Equine 6-Plex ELISA Kits-BMP

GR225025 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-CCL

GR225026 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-MMP

GR225027 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-TLR

GR225028 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-Hormones

GR225029 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-SAA

GR225030 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-S100

GR225031 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-CXCL

GR225032 96-well
EUR 629

Nori® Canine 3-Plex ELISA Kits-Interferons

GR226009 96-well
EUR 503

Nori® Canine 4-Plex ELISA Kits-Immunoglobulins

GR226017 96-well
EUR 545

Nori® Canine 6-Plex ELISA Kits-BMP

GR226025 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-CCL

GR226026 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-MMP

GR226027 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-TLR

GR226028 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-Hormones

GR226029 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-SAA

GR226030 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-S100

GR226031 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-CXCL

GR226032 96-well
EUR 629

Nori® Bovine 3-Plex ELISA Kits-Interferons

GR228009 96-well
EUR 503

Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins

GR228017 96-well
EUR 545

Nori® Bovine 6-Plex ELISA Kits-BMP

GR228025 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-CCL

GR228026 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-MMP

GR228027 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-TLR

GR228028 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-Hormones

GR228029 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-SAA

GR228030 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-S100

GR228031 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-CXCL

GR228032 96-well
EUR 629

Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody

20-abx116620
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258)

OKSA11258 1 u
EUR 1236
Description: Description of target: RABBIT ANTI 14-3-3 ISOFORM PANEL;Species reactivity: Sheep;Application: ELISA, IHC-FFPE, WB;Assay info: ;Sensitivity:

Nori® Porcine 6-Plex ELISA Kits-Growth Factors

GR224024 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-Inflammation Panel

GR224033 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-Cardiac Panel

GR224034 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-Growth Factors

GR225024 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-Inflammation Panel

GR225033 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-Cardiac Panel

GR225034 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-Growth Factors

GR226024 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-Inflammation Panel

GR226033 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-Cardiac Panel

GR226034 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-Growth Factors

GR228024 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-Inflammation Panel

GR228033 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-Cardiac Panel

GR228034 96-well
EUR 629

Nori® Porcine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR224001 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR224002 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR224003 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR224004 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR224005 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR224006 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR224008 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR225001 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR225002 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR225003 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR225004 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR225005 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR225006 96-well
EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR225008 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR226001 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR226002 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR226003 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR226004 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR226005 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR226006 96-well
EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR226008 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1A-IL8-TNFA

GR228001 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL8-TNFA

GR228002 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL6-TNFA

GR228003 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL10-TNFA

GR228004 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL6-IL8-TNFA

GR228005 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL6-IL10-TNFA

GR228006 96-well
EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL2-IL4

GR228008 96-well
EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL4-IL6-IL-10

GR224007 96-well
EUR 503

Nori® Porcine 4-Plex ELISA Kits-IL1B-IL4-IL10-TNFA

GR224013 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL6-IL8-IL10-TNFA

GR224014 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL4-IL6-IL10-TNFA

GR224015 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL1B-IL2-IL4-IL10

GR224018 96-well
EUR 545

Nori® Porcine 6-Plex ELISA Kits-Type I Cytokine Panel

GR224035 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-Type II Cytokine Panel

GR224036 96-well
EUR 629

Nori® Equine 3-Plex ELISA Kits-IL4-IL6-IL-10

GR225007 96-well
EUR 503

Nori® Equine 4-Plex ELISA Kits-IL1B-IL4-IL10-TNFA

GR225013 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL6-IL8-IL10-TNFA

GR225014 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL4-IL6-IL10-TNFA

GR225015 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL1B-IL2-IL4-IL10

GR225018 96-well
EUR 545

Nori® Equine 6-Plex ELISA Kits-Type I Cytokine Panel

GR225035 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-Type II Cytokine Panel

GR225036 96-well
EUR 629

Nori® Canine 3-Plex ELISA Kits-IL4-IL6-IL-10

GR226007 96-well
EUR 503

Nori® Canine 4-Plex ELISA Kits-IL1B-IL4-IL10-TNFA

GR226013 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL6-IL8-IL10-TNFA

GR226014 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL4-IL6-IL10-TNFA

GR226015 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL1B-IL2-IL4-IL10

GR226018 96-well
EUR 545

Nori® Canine 6-Plex ELISA Kits-Type I Cytokine Panel

GR226035 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-Type II Cytokine Panel

GR226036 96-well
EUR 629

Nori® Bovine 3-Plex ELISA Kits-IL4-IL6-IL-10

GR228007 96-well
EUR 503

Nori® Bovine 4-Plex ELISA Kits-IL1B-IL4-IL10-TNFA

GR228013 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL6-IL8-IL10-TNFA

GR228014 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL4-IL6-IL10-TNFA

GR228015 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL1B-IL2-IL4-IL10

GR228018 96-well
EUR 545

Nori® Bovine 6-Plex ELISA Kits-Type I Cytokine Panel

GR228035 96-well
EUR 629

Nori® Bovine 6-Plex ELISA Kits-Type II Cytokine Panel

GR228036 96-well
EUR 629

Human Anti-Chikungunya virus (CHIKV) IgM capture ELISA kits, 96 tests, qualitative

910-705-CHM 1 Kit
EUR 834

Nori® Porcine 4-Plex ELISA Kits-IL1A-IL-6-IL8-TNFA

GR224010 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL1B-IL-6-IL8-TNFA

GR224011 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL1B-IL6-IL-10-TNFA

GR224012 96-well
EUR 545

Nori® Porcine 4-Plex ELISA Kits-IL4-IL6-IL-10-IL12

GR224016 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL1A-IL-6-IL8-TNFA

GR225010 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL1B-IL-6-IL8-TNFA

GR225011 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL1B-IL6-IL-10-TNFA

GR225012 96-well
EUR 545

Nori® Equine 4-Plex ELISA Kits-IL4-IL6-IL-10-IL12

GR225016 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL1A-IL-6-IL8-TNFA

GR226010 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL1B-IL-6-IL8-TNFA

GR226011 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL1B-IL6-IL-10-TNFA

GR226012 96-well
EUR 545

Nori® Canine 4-Plex ELISA Kits-IL4-IL6-IL-10-IL12

GR226016 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL1A-IL-6-IL8-TNFA

GR228010 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL1B-IL-6-IL8-TNFA

GR228011 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL1B-IL6-IL-10-TNFA

GR228012 96-well
EUR 545

Nori® Bovine 4-Plex ELISA Kits-IL4-IL6-IL-10-IL12

GR228016 96-well
EUR 545

Human Homocystein CLIA(Homocystein CLIA) ELISA Kit

QY-E05403 96T
EUR 361

Nori® Porcine 6-Plex ELISA Kits-IL1A-IL2-IL4-IL6-IL8-IL10

GR224019 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-IL1B-IL2-IL4-IL6-IL8-IL10

GR224020 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL8-IL10-IL12

GR224021 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL8-IL10-TNFA

GR224022 96-well
EUR 629

Nori® Porcine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL10-TNFA-CRP

GR224023 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-IL1A-IL2-IL4-IL6-IL8-IL10

GR225019 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-IL1B-IL2-IL4-IL6-IL8-IL10

GR225020 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL8-IL10-IL12

GR225021 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL8-IL10-TNFA

GR225022 96-well
EUR 629

Nori® Equine 6-Plex ELISA Kits-IL1B-IL4-IL6-IL10-TNFA-CRP

GR225023 96-well
EUR 629

Nori® Canine 6-Plex ELISA Kits-IL1A-IL2-IL4-IL6-IL8-IL10

GR226019 96-well
EUR 629


In the last months of 2019, an outbreak of fatal respiratory disease that began in Wuhan, China, and quickly spread to other parts of the world. It named COVID-19, and to date, thousands of cases of infection and deaths reported worldwide. The disease is associated with a variety of symptoms that make it difficult to diagnose accurately.

Previous SARS pandemic in 2003, researchers found that patients with fever, cough, or sore throat has influenza virus-positive rate of 5%. These findings triggered in our minds that the various symptoms and also the relatively high prevalence of death in our patients may be due to co-infection with other viruses.