Influenza is a major respiratory viral illness caused by infection of influenza A virus (IAV), which still persists in various global seasonal epidemics every year. the host immune response is a key factor that determines the severity of influenza infection, present an attractive target for the development of new therapies for the treatment.
Among the double track signal transduction that regulates the activation of the host immune response and function in response to infection IAV, the mitogen-activated protein kinase (MAPK) pathway that wicks important signaling, downstream receptors pattern recognition (PRRS), activated by IAVs that regulate various processes cell in the immune cells of both the innate and adaptive immunity.
In addition, aberrant activation of MAPK overexuberant underlying the production of inflammatory mediators, promoting the development of a “cytokine storm”, the characteristics of severe respiratory viral diseases. Therefore, explanation of MAPK regulatory role in the immune response to IAVs not only important for understanding the pathogenesis of severe influenza, but it is also important to develop a MAPK-dependent therapies for the treatment of respiratory viral diseases. In this review, we will summarize the current understanding of the function of MAPK in both the innate and adaptive immune responses to IAVs and discuss their contribution to the cytokine storm caused by the highly pathogenic influenza virus.
recombinant influenza A virus hemagglutinins (HA) and tetrameric neuraminidases (NA) has proven to be an excellent tool to decipher biological properties. Receptor binding and cleavage of sialic acid by a recombinant protein correlated satisfactory compared with the whole virus. Expression of HA and NA can be achieved in many different laboratories host.
For the study of immunology and receptor interactions, however, insect and mammalian cell expressed proteins are preferred because of the presence of N-linked glycosylation and disulfide bond formation. Because the expression of mammalian cells is widely applied, yield enhancement expression is an important goal. Here we report that the use of codon-optimized genes and fusion sfGFP, HA expression results can be improved
Regulation of Host Immune Responses against Influenza A Virus Infection by Mitogen-Activated Protein Kinases (MAPKs)
Comparative Analysis of Antiviral Activity of IgG and IgA antibodies to Influenza A Virus M2 Protein
The influenza A virus (IAV) matrix-2 (M2) protein is a viral envelope proteins conserved antigens that plays an important role in virus starters together with other envelope proteins, hemagglutinin (HA). M2-specific mouse monoclonal antibody IgG, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be non-neutralizing antibodies, but inhibits plaque formation IAV strains.
In this study, we produce rM2ss23 chimeric (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activity. Using gel chromatography, ch-rM2ss23 IgA is divided into three subsets of antibodies: IgA monomer (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t / q-IgA).
Basic Cytotoxicity Test Kits
CT17001
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125 Tests
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CT17002
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250 Tests
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125 Tests
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KF17372
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HISTAR DETECTION SYSTEM Kits (OKSA11259)
OKSA11259
Aviva Systems Biology
50 Tests
EUR 678
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:
HISTAR DETECTION SYSTEM Kits (OKSA11260)
OKSA11260
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150 Tests
EUR 1602
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HISTAR DETECTION SYSTEM Kits (OKSA11261)
OKSA11261
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500 Tests
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MitoPT™ TMRE & TMRM Kits
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500 Test
EUR 574.8
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500 Test
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Rat Neural Stem Cell Kits
PC37123
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1 X 10(6) *minimum order 6 vials*
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MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)
OKSA11298
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500 Tests
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MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)
OKSA11300
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JBScreen Membrane 1-4 - All 4 kits
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XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)
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Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody
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Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258)
OKSA11258
Aviva Systems Biology
1 u
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GR224033
Genorise Scientific
96-well
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GR225033
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Genorise Scientific
96-well
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Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)
CBA-200
Cell Biolabs
50 assays
EUR 672
Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment.
Proteasome Assay Kit
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Glutathione Assay Kit
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HDAC Assay Kit
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100 assays
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HDAC Assay Kit
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HAT Assay Kit
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100 assays
EUR 888
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SOD Assay Kit
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100 assays
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100 assays
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100 assays
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100 assays
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100 assays
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100 assays
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100 assays
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100 assays
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55R-1486
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55R-1492
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100 assays
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55R-1526
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100 assays
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55R-1528
Fitzgerald
100 assays
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We found that t / q-IgA have significantly higher capacity to reduce plaque size of IAVs of m-IgG and IgA, most likely caused by a decrease in the number of offspring produced virus particles from infected cells. Interestingly, HA-M2 colocalization was greatly reduced on the surface of infected cells with antibodies ch-rM2ss23. These results indicate that the polymeric IgA anti-M2 Limit beginner IAV more efficient than IgG and suggested the role of anti-M2 IgA cross-protective immunity IAVs.
