Regulation of Host Immune Responses against Influenza A Virus Infection by Mitogen-Activated Protein Kinases (MAPKs)

Influenza is a major respiratory viral illness caused by infection of influenza A virus (IAV), which still persists in various global seasonal epidemics every year. the host immune response is a key factor that determines the severity of influenza infection, present an attractive target for the development of new therapies for the treatment.

Among the double track signal transduction that regulates the activation of the host immune response and function in response to infection IAV, the mitogen-activated protein kinase (MAPK) pathway that wicks important signaling, downstream receptors pattern recognition (PRRS), activated by IAVs that regulate various processes cell in the immune cells of both the innate and adaptive immunity.

In addition, aberrant activation of MAPK overexuberant underlying the production of inflammatory mediators, promoting the development of a “cytokine storm”, the characteristics of severe respiratory viral diseases. Therefore, explanation of MAPK regulatory role in the immune response to IAVs not only important for understanding the pathogenesis of severe influenza, but it is also important to develop a MAPK-dependent therapies for the treatment of respiratory viral diseases. In this review, we will summarize the current understanding of the function of MAPK in both the innate and adaptive immune responses to IAVs and discuss their contribution to the cytokine storm caused by the highly pathogenic influenza virus.


Trimeric soluble recombinant influenza A virus hemagglutinins (HA) and tetrameric neuraminidases (NA) has proven to be an excellent tool to decipher biological properties. Receptor binding and cleavage of sialic acid by a recombinant protein correlated satisfactory compared with the whole virus. Expression of HA and NA can be achieved in many different laboratories host.

For the study of immunology and receptor interactions, however, insect and mammalian cell expressed proteins are preferred because of the presence of N-linked glycosylation and disulfide bond formation. Because the expression of mammalian cells is widely applied, yield enhancement expression is an important goal. Here we report that the use of codon-optimized genes and fusion sfGFP, HA expression results can be improved

Regulation of Host Immune Responses against Influenza A Virus Infection by Mitogen-Activated Protein Kinases (MAPKs)
Regulation of Host Immune Responses against Influenza A Virus Infection by Mitogen-Activated Protein Kinases (MAPKs)

Comparative Analysis of Antiviral Activity of IgG and IgA antibodies to Influenza A Virus M2 Protein

The influenza A virus (IAV) matrix-2 (M2) protein is a viral envelope proteins conserved antigens that plays an important role in virus starters together with other envelope proteins, hemagglutinin (HA). M2-specific mouse monoclonal antibody IgG, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be non-neutralizing antibodies, but inhibits plaque formation IAV strains.

In this study, we produce rM2ss23 chimeric (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activity. Using gel chromatography, ch-rM2ss23 IgA is divided into three subsets of antibodies: IgA monomer (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t / q-IgA).

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)

30007 1KIT
EUR 283.2
Description: Minimum order quantity: 1 unit of 1KIT

ADA Assay Kit

abx098403-Hitachi7060R190ml2R290ml1 Hitachi 7060; R1: 90ml×2 R2: 90ml×1
EUR 886.8

ADA Assay Kit

abx098403-Hitachi7170R140ml4R220ml4 Hitachi 7170; R1: 40ml×4 R2: 20ml×4
EUR 961.2

ADA Assay Kit

abx098403-Hitachi7170R160ml4R260ml2 Hitachi 7170; R1: 60ml×4 R2: 60ml×2
EUR 1093.2

ADA Assay Kit

abx098403-Toshiba40R150ml4R250ml2 Toshiba 40; R1: 50ml×4 R2: 50ml×2
EUR 943.2

RET Assay Kit

79566 96 rxns.
EUR 535
Description: RET is a receptor kinase for GDNF (glial cell-line derived neurotrophic factor) family ligands (GFLs). Activation of wild-type RET is important in several cancers where it contributes to tumor progression through various mechanisms. In addition, it has been shown that activating mutation as well as RET rearrangement that leads constitutively active protein plays a significant role in various cancer types. Importantly, small molecule inhibitors of RET have been clinically proved as a promising therapeutic reagent in medullary thyroid cancer and are being evaluated for other types of cancers related to RET overexpression or mutation. The RET Assay Kit is designed to measure RET activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The RET Assay Kit comes in a convenient 96-well format, with enough purified recombinant RET enzyme, RET substrate peptide (IRF-1Rtide), ATP and kinase assay buffer for 100 enzyme reactions.

BTK Assay Kit

79568 96 rxns.
EUR 560
Description: Bruton's tyrosine kinase or BTK, is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. The BTK Assay Kit is designed to measure BTK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The BTK Assay Kit comes in a convenient 96-well format, with enough purified recombinant BTK enzyme, PolyGluTyr peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

SYK Assay Kit

79671 96 rxns.
EUR 525
Description: SYK of the nonreceptor tyrosine kinases plays a role in autoimmune diseases like Epstein Barr virus and hematopoietic malignancies. The SYK Assay Kit is designed to measure SYK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

SRC Assay Kit

79680 96 rxns.
EUR 535
Description: SRC is a member of the nonreceptor tyrosine kinases that plays a role in many cellular functions, including cell adhesion, growth, and differentiation. SRC has been implicated in diseases such as chronic kidney disease and metastatic bone disease. The SRC Assay Kit is designed to measure SRC activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The SRC Assay Kit comes in a convenient 96-well format, with enough purified recombinant SRC enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

LCK Assay Kit

79794 96 rxns.
EUR 535
Description: The LCK Assay Kit is designed to measure LCK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

POP Assay Kit

80106 96 rxns.
EUR 685
Description: The Fluorogenic Prolyl OligoPeptidase (POP)_x000D_ Assay Kit is a complete assay system designed to measure activity of the purified POP_x000D_ enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive_x000D_ materials and chromatography in traditional assays. Purified human recombinant POP is_x000D_ included in the kit as a positive control. Using this kit, only one simple step, in which the_x000D_ fluorometric substrate is incubated with purified POP, is needed to analyze the POP_x000D_activity level. The resulting fluorescent product can then be easily measured with a_x000D_ microtiter-plate fluorimeter.

FAP Assay Kit

80210 96 rxns.
EUR 1175
Description: The Fluorogenic FAP Assay Kit is designed to measure FAP activity using purified_x000D_FAP for screening and profiling applications. _x000D_The key to the Fluorogenic FAP Assay Kit is the fluorogenic substrate. Using this kit,_x000D_only one simple step on a microtiter plate is required for FAP reactions. The DPP_x000D_fluorometric substrate is incubated with a sample containing FAP enzyme to produce a_x000D_fluorophore that can then be measured using a fluorescence reader.

FYN Assay Kit

78003 96 rxns.
EUR 535
Description: The FYN Assay Kit is designed to measure FYN activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

ADA Assay Kit

abx090675-100tests 100 tests
EUR 284.4

DNA Assay Kit

6023 1 kit
EUR 216.6
Description: DNA Assay Kit

HAT Assay Kit

55R-1373 100 assays
EUR 888
Description: Assay Kit for detection of HAT in the research laboratory

SOD Assay Kit

55R-1374 100 assays
EUR 722.4
Description: Assay Kit for detection of SOD in the research laboratory

ATP Assay Kit

55R-1380 100 assays
EUR 970.8
Description: Assay Kit for detection of ATP in the research laboratory

ADP Assay Kit

55R-1381 100 assays
EUR 970.8
Description: Assay Kit for detection of ADP in the research laboratory

FAD Assay Kit

55R-1382 100 assays
EUR 878.4
Description: Assay Kit for detection of FAD in the research laboratory

PEP Assay Kit

55R-1384 100 assays
EUR 1051.2
Description: Assay Kit for detection of PEP in the research laboratory

JNK Assay Kit

55R-1407 40 assays
EUR 1041.6
Description: Assay Kit for detection of JNK in the research laboratory

JNK Assay Kit

55R-1408 40 assays
EUR 1174.8
Description: Assay Kit for detection of JNK in the research laboratory

Akt Assay Kit

55R-1409 40 assays
EUR 1174.8
Description: Assay Kit for detection of Akt in the research laboratory

MDA Assay Kit

55R-1512 100 assays
EUR 970.8
Description: Assay Kit for detection of MDA in the research laboratory

PAH Assay Kit

55R-1547 100 assays
EUR 888
Description: Assay Kit for detection of PAH in the research laboratory

DNA Assay Kit

Z5030023 250 assays
EUR 636

ATP Assay Kit

Z5030041 100 assays
EUR 636

Iron Assay Kit

abx098439-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1:40ml×1 R2:10ml×1
EUR 247.2

Iron Assay Kit

abx098439-Hitachi7170R140ml1R210ml1 Hitachi 7170; R1:40ml×1 R2:10ml×1
EUR 247.2

Iron Assay Kit

abx098439-Hitachi7170R160ml1R215ml1 Hitachi 7170; R1:60ml×1 R2:15ml×1
EUR 266.4

Iron Assay Kit

abx098439-Toshiba40R140ml1R210ml1 Toshiba 40; R1:40ml×1 R2:10ml×1
EUR 247.2

NGAL Assay Kit

abx098449-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1: 40ml×1 R2: 10ml×1
EUR 1130.4

NGAL Assay Kit

abx098449-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 679.2

NGAL Assay Kit

abx098449-Hitachi7170R140ml1R210ml1 Hitachi 7170; R1: 40ml×1 R2: 10ml×1
EUR 1130.4

NGAL Assay Kit

abx098449-Toshiba120R140ml1R210ml1 Toshiba 120; R1: 40ml×1 R2: 10ml×1
EUR 1130.4

NGAL Assay Kit

abx098449-Toshiba40R140ml1R210ml1 Toshiba 40; R1: 40ml×1 R2: 10ml×1
EUR 1130.4

Urea Assay Kit

abx098464-Hitachi7020R150ml4R250ml1 Hitachi 7020; R1: 50ml×4 R2: 50ml×1
EUR 266.4

Urea Assay Kit

abx098464-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 266.4

Urea Assay Kit

abx098464-Toshiba120R140ml4R240ml1 Toshiba 120; R1: 40ml×4 R2: 40ml×1
EUR 266.4

AMBP Assay Kit

abx098467-Hitachi7060R145ml1R215ml1 Hitachi 7060; R1: 45ml×1 R2: 15ml×1
EUR 585.6

AMBP Assay Kit

abx098467-Hitachi7060R190ml2R230ml2 Hitachi 7060; R1: 90ml×2 R2: 30ml×2
EUR 622.8

AMBP Assay Kit

abx098467-Hitachi7170R145ml1R215ml1 Hitachi 7170; R1: 45ml×1 R2: 15ml×1
EUR 585.6

AMBP Assay Kit

abx098467-Toshiba40R145ml1R215ml1 Toshiba 40; R1: 45ml×1 R2: 15ml×1
EUR 585.6

TrkA Assay Kit

79548 96 rxns.
EUR 535
Description: The tropomyosin receptor kinases (TRK) are a family of tyrosine receptor kinases, which include TrkA, TrkB and TrkC. TrkA is activated by binding to nerve growth factor (NGF), resulting in activation of cell proliferation through the RAS/MAPK/ERK and PLCγ/PI3K pathways. Constitutive activation of TrkA is associated with colorectal cancer, suggesting TrkA inhibitors may have potential therapeutic value. The TrkA Assay Kit is designed to measure TrkA activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The TrkA Assay Kit comes in a convenient 96-well format, with enough purified recombinant TrkA enzyme, TrkA substrate, ATP and kinase assay buffer for 100 enzyme reactions.

CDK1 Assay Kit

79597 96 rxns.
EUR 535
Description: Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. CDKs are active only when bound to their regulator proteins, cyclins. CDK activity is tightly controlled for successful cell division. Since abnormal cell division represents cancer pathology, controlling CDK activity has been shown as a promising therapeutic strategy. In particular, CDK1 plays an important role in mitotic progression. The CDK1 Assay Kit is designed to measure CDK1/CyclinB1 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

CDK2 Assay Kit

79599 96 rxns.
EUR 535
Description: Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. CDKs are active only when bound to their regulator proteins, cyclins. CDK activity is tightly controlled for successful cell division. Since abnormal cell division represents cancer pathology, controlling CDK activity has been shown as a promising therapeutic strategy. In particular, CDK2 plays an important role in DNA replication. The CDK2 Assay Kit is designed to measure CDK2/CyclinA2 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

CDK5 Assay Kit

79600 96 rxns.
EUR 535
Description: CDK5 is an unconventional member of the CDK family. It is expressed in post-mitotic neurons, and its activity is regulated by neuron-specific activators p35/p25 and p39 rather than the cyclins. It has been suggested that CDK5 plays several roles in the nervous system, including Tau aggregation and tangle formation. The CDK5 Assay Kit is designed to measure CDK5 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

CDK7 Assay Kit

79603 96 rxns.
EUR 535
Description: Cyclin-dependent kinase 7 (CDK7) assembled with Cyclin H and MAT1 comprises an important sub-component of the transcription factor TFIIH. It is known to have dual roles, which include transcription regulation via phosphorylating the C-terminal domain (CTD) of RNA polymerase II as well as controlling cell cycle progression as a CDK activating kinase (CAK). The CDK7 Assay Kit is designed to measure CDK7/Cyclin H/MAT1 activity for screening and profiling applications, using ADP-Glo® as a detection reagent.

CDK9 Assay Kit

79628 96 rxns.
EUR 575
Description: Cyclin-dependent kinase 9 (CDK9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb), which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. The CDK9 Assay Kit is designed to measure CDK9/CyclinT activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent. The CDK9 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK9/CyclinT enzyme, CDK substrate peptide 2, ATP and kinase assay buffer for 100 enzyme reactions.

CDK4 Assay Kit

79674 96 rxns.
EUR 550
Description: Cyclin-dependent kinase 4 (CDK4) assembles with the "D" type of Cyclins, e.g. cyclins D1, D2, and D3, and play a pivotal role in the cell cycle entering the S phase. Dysregulation of the activity of CDK4 is related to many different cancers, indicating CDK4 inhibitors as promising anticancer treatments. In fact, multiple CDK4 inhibitors, including Palbociclib, were approved by the FDA recently. The CDK4 Assay Kit is designed to measure CDK4/CyclinD3 activity for screening and profiling applications, using Kinase-Glo® Max as a detection reagent.

YES1 Assay Kit

79681 96 rxns.
EUR 535
Description: Proto-oncogene tyrosine-protein kinase YES1 has been implicated in regulation of cell growth and survival, apoptosis, cell-cell adhesion, and differentiation. It has been identified as a potential target in basal-like breast cancers. The YES1 Assay Kit is designed to measure YES1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The YES1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant YES1 enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

HPK1 Assay Kit

79775 96 rxns.
EUR 560
Description: The HPK1 Assay Kit is designed to measure HPK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

cKIT Assay Kit

79815 96 rxns.
EUR 535
Description: The cKIT Assay Kit is designed to measure cKIT activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

TBK1 Assay Kit

79871 96 rxns.
EUR 510
Description: The TBK1 Assay Kit is designed to measure TBK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

PIM2 Assay Kit

79883 96 rxns.
EUR 535
Description: The PIM2 Assay Kit is designed to measure PIM2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

PIM1 Assay Kit

79885 96 rxns.
EUR 560
Description: The PIM1 Assay Kit is designed to measure PIM1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

PIM3 Assay Kit

79886 96 rxns.
EUR 535
Description: The PIM3 Assay Kit is designed to measure PIM3 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

ACLY Assay Kit

79904 96 rxns.
EUR 590
Description: ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. The ACLY Assay Kit is designed to measure ACLY activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. 

Wee1 Assay Kit

79909 96 rxns.
EUR 560
Description: The Wee1 Assay Kit is designed to measure Wee1 activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. 

DPP3 Assay Kit

80203 96 rxns.
EUR 615
Description: The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP3 reactions; no time-consuming washing steps are required. The fluorometric substrate is incubated with a sample containing DPP3 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

DPP4 Assay Kit

80204 96 rxns.
EUR 465
Description: The Fluorogenic DPP4 Assay Kit is_x000D_designed to measure DPP4 activity using purified DPP4 for screening and profiling applications._x000D_ The key to the Fluorogenic DPP4 Assay Kit_x000D_is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter_x000D_plate is required for DPP4 reactions; no time-consuming washing steps are required. The DPP fluorometric substrate is incubated with a_x000D_sample containing DPP4 enzyme to produce a fluorophore that can then be measured_x000D_using a fluorescence reader._x000D_

DPP7 Assay Kit

80207 96 rxns.
EUR 515
Description: The Fluorogenic DPP7 Assay Kit is_x000D_designed to measure DPP7 activity using purified DPP7 for screening and profiling applications._x000D_The key to the Fluorogenic DPP7 Assay Kit is the_x000D_specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is_x000D_required for DPP7 reactions. The fluorometric substrate is incubated with a sample_x000D_containing DPP7 enzyme to produce a fluorophore that can then be measured using a_x000D_fluorescence reader.

DPP8 Assay Kit

80208 96 rxns.
EUR 515
Description: The Fluorogenic DPP8_x000D_Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and_x000D_profiling applications. The key to_x000D_the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one_x000D_simple step on a microtiter plate is required for DPP8 reactions. The fluorometric_x000D_substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore_x000D_that can then be measured using a fluorescence reader._x000D_

DPP9 Assay Kit

80209 96 rxns.
EUR 615
Description: The Fluorogenic_x000D_DPP9 Assay Kit is designed to measure DPP9 activity using purified DPP9 for screening_x000D_and profiling applications. The_x000D_key to the Fluorogenic DPP9 Assay Kit is the fluorogenic substrate. Using this kit,_x000D_only one simple step on a microtiter plate is required for DPP9 reactions. The fluorometric_x000D_substrate is incubated with a sample containing DPP9 enzyme to produce a_x000D_fluorophore that can then be measured using a fluorescence reader.

HER4 Assay Kit

78005 96 rxns.
EUR 535
Description: The HER4 Assay Kit is designed to measure HER4 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

AKT1 Assay Kit

78038 96 rxns.
EUR 535
Description: The AKT1 Assay Kit is designed to measure AKT1 activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. 

HDAC Assay Kit

55R-1371 100 assays
EUR 807.6
Description: Assay Kit for detection of HDAC in the research laboratory

HDAC Assay Kit

55R-1372 100 assays
EUR 807.6
Description: Assay Kit for detection of HDAC in the research laboratory

cAMP Assay Kit

55R-1389 100 assays
EUR 847.2
Description: Assay Kit for detection of cAMP activity in the research laboratory

cGMP Assay Kit

55R-1390 100 assays
EUR 847.2
Description: Assay Kit for detection of cGMP activity in the research laboratory

Urea Assay Kit

55R-1391 100 assays
EUR 888
Description: Assay Kit for detection of Urea in the research laboratory

Zinc Assay Kit

55R-1394 100 assays
EUR 807.6
Description: Assay Kit for detection of Zinc in the research laboratory

Iron Assay Kit

55R-1395 100 assays
EUR 909.6
Description: Assay Kit for detection of Iron in the research laboratory

Heme Assay Kit

55R-1492 100 assays
EUR 807.6
Description: Assay Kit for detection of Heme in the research laboratory

DPP4 Assay Kit

55R-1526 100 assays
EUR 900
Description: Assay Kit for detection of DPP4 in the research laboratory

MMP3 Assay Kit

55R-1530 100 assays
EUR 867.6
Description: Assay Kit for detection of MMP3 in the research laboratory

Iron Assay Kit

abx298872-100Assays 100 Assays
EUR 510

Zinc Assay Kit

abx298876-100Assays 100 Assays
EUR 698.4

Urea Assay Kit

STA-382 192 assays
EUR 762
Description: Cell Biolabs? Urea Assay Kit is based on the Berthelot reaction.  Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm.  Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.

Urea Assay Kit

Z5030016 500 assays
EUR 558

Iron Assay Kit

Z5030022 250 assays
EUR 668

Heme Assay Kit

Z5030027 250 assays
EUR 739

Zinc Assay Kit

Z5030036 250 assays
EUR 1106

PDE6C Assay Kit

79501 96 rxns.
EUR 405
Description: The PDE6C Assay Kit is designed for identification of PDE6C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE6C to the binding agent.Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The PDE6C inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE6C enzyme, fluorescently labeled PDE6 substrate (cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE6C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE6C reactions. First, the fluorescently labeled cGMP is incubated with PDE6C for 1 hour. Second, the binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization._x000D_

GSK3α Assay Kit

79698 96 rxns.
EUR 535
Description: The GSK3α Assay Kit is designed to measure GSK3α activity for screening and profiling applications using Kinase-Glo® (Promega) as a detection reagent.

GSK3β Assay Kit

79700 96 rxns.
EUR 590
Description: The GSK3β Assay Kit is designed to measure GSK3β activity for screening and profiling applications using Kinase-Glo® (Promega) as a detection reagent.

PDE3A Assay Kit

79736 96 rxns.
EUR 425
Description: The PDE3A Assay Kit is designed for identification of PDE3A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3A to the binding agent. REPLACES #60330

FGFR2 Assay Kit

79804 96 rxns.
EUR 535
Description: The FGFR2 Assay Kit is designed to measure FGFR2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

FGFR4 Assay Kit

79807 96 rxns.
EUR 535
Description: The FGFR4 Assay Kit is designed to measure FGFR4 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Renin Assay Kit

80211 96 rxns.
EUR 450
Description: The Renin Assay Kit is designed to_x000D_measure Renin activity for screening and profiling applications. It comes in a convenient_x000D_96-well format, with purified, activated renin, renin substrate, and assay buffer for 100_x000D_enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin_x000D_inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this_x000D_kit, only one simple step on a microtiter plate is required for renin reactions. The_x000D_fluorometric substrate is incubated with a sample containing renin enzyme to produce a_x000D_fluorophore that can then be measured using a fluorescence reader.

EPHA2 Assay Kit

78002 96 rxns.
EUR 535
Description: The EPHA2 Assay Kit is designed to measure EPHA2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

SPHK1 Assay Kit

78026 96 rxns.
EUR 535
Description: The SPHK1 Assay Kit is designed to measure SPHK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

BACE1 Assay Kit

71656 96 rxns.
EUR 530
Description: Bace1 (β-secretase 1) is an aspartic protease that is involved in the processing of the Amyloid precursor protein (APP). Cleavage of APP by BACE1 followed by γ-secretase results in β-amyloid peptide production, which ultimately leads toxic Aβ accumulation. In Alzheimer's disease (AD), it has been widely accepted that Aβ aggregation plays a critical role in AD pathogenesis, suggesting that BACE1 could be a potential target to treat AD. The BACE1 FRET Assay Kit is designed to measure BACE1 activity for screening and profiling applications based on fluorescence resonance energy transfer (FRET) using a labeled peptide substrate (below).

HSP70 Assay Kit

78414 96 rxns.
EUR 450
Description: The HSP70 Assay Kit is a chemiluminescence assay kit designed to measure HSP70 adenosine triphosphate (ATP) hydrolysis activity for screening and profiling applications using ADP-Glo® Assay as a detection reagent.

PDE1A Assay Kit

60310 96 rxns.
EUR 405
Description: The PDE1A Assay Kit is designed for identification of PDE1A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1A1 to the binding agent. The key to the PDE1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader._x000D_

PDE1B Assay Kit

60311 96 rxns.
EUR 405
Description: The PDE1B Assay Kit is designed for identification of PDE1B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1B to the binding agent. The key to the PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1B for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then be_x000D_measured using a fluorescence reader equipped for the measurement of fluorescence_x000D_polarization.

PDE1C Assay Kit

60312 96 rxns.
EUR 405
Description: The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction_x000D_mix to produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader.

PDE2A Assay Kit

60320 96 rxns.
EUR 405
Description: The PDE2A Assay Kit is_x000D_designed for identification of PDE2A1 inhibitors using fluorescence polarization. The_x000D_assay is based on the binding of a fluorescent nucleotide monophosphate generated by_x000D_PDE2A1 to the binding agent. The key to the PDE2A Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE2A1 reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE2A1 for 1 hour. Second, binding agent is added to the reaction mix to_x000D_produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence readerequipped for the measurement of fluorescence polarization._x000D_

PDE2A Assay Kit

60321 384 rxns.
EUR 800
Description: The PDE2A Assay Kit is designed for identification of PDE2A inhibitors using_x000D_fluorescence polarization.The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE2A to the binding agent. PDE2A catalyzes the hydrolysis of_x000D_the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP)._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization_x000D_of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating_x000D_nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized_x000D_light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain_x000D_random orientations and emit light with low polarization. The key to the PDE2A Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE2A_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE2A1_x000D_for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in_x000D_fluorescent polarization that can then be measured using a fluorescence reader.

PDE3B Assay Kit

60331 96 rxns.
EUR 425
Description: The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to_x000D_produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader equipped for the measurement of fluorescence polarization.

PDE4A Assay Kit

60340 96 rxns.
EUR 425
Description: The PDE4A Assay Kit is designed for identification of PDE4A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4A to the binding agent. The key to the_x000D_PDE4A1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on_x000D_a microtiter plate are required for PDE4A1A reactions. First, the fluorescently labeled_x000D_cAMP is incubated with a sample containing PDE4A1A for 1 hour. Second, a binding_x000D_agent is added to the reaction mix to produce a change in fluorescent polarization that_x000D_can then be measured using a fluorescence reader equipped for the measurement of_x000D_fluorescence polarization.

PDE7A Assay Kit

60370 96 rxns.
EUR 405
Description: The PDE7A Assay Kit is designed_x000D_for identification of PDE7A inhibitors using fluorescence polarization. The assay is_x000D_based on the binding of a fluorescent nucleotide monophosphate generated by PDE7A_x000D_to the binding agent. The key to the PDE7A_x000D_Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE7A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE7A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader.

PDE7B Assay Kit

60371 96 rxns.
EUR 405
Description: The PDE7B Assay Kit is designed for identification of PDE7B inhibitors using fluorescence_x000D_polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE7B to the binding agent. The key to the PDE7B Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE7B reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE7B for 1 hour. Second, a binding agent is added to the reaction_x000D_mix to produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader.

PDE7A Assay Kit

60373 384 rxns.
EUR 800
Description: The PDE7A Assay Kit is designed for identification of PDE7A inhibitors using_x000D_fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE7A to the binding agent. PDE7A catalyzes the hydrolysis of_x000D_the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP)._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization_x000D_of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating_x000D_nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized_x000D_light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain_x000D_random orientations and emit light with low polarization. The key to the PDE7A Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE7A_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE7A_x000D_for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in_x000D_fluorescent polarization that can then be measured using a fluorescence reader.

PDE8A Assay Kit

60380 96 rxns.
EUR 405
Description: The PDE8A Assay Kit is designed for identification of PDE8A inhibitors_x000D_using fluorescence polarization. The assay is based on the binding of a fluorescent_x000D_nucleotide monophosphate generated by PDE8A to the binding agent. The key to the PDE8A_x000D_Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE8A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE8A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader.

PDE9A Assay Kit

60381 96 rxns.
EUR 405
Description: The PDE9A_x000D_Assay Kit is designed for identification of PDE9A inhibitors using fluorescence_x000D_polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE9A to the binding agent. The key to the PDE9A Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE9A reactions. First, the fluorescently labeled cGMP is incubated with a_x000D_sample containing PDE9A for 1 hour. Second, a binding agent is added to the reaction_x000D_mix to produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader._x000D_

PDE1C Assay Kit

60382 384 rxns.
EUR 800
Description: The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

PDE3B Assay Kit

60383 384 rxns.
EUR 800
Description: The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE4C Assay Kit

60384 384 rxns.
EUR 800
Description: The PDE4C1 Assay Kit is designed for identification of PDE4C1 inhibitors_x000D_using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE4C1 to the binding agent. The key to the PDE4C1 Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for_x000D_PDE4C1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing_x000D_PDE4C1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change_x000D_in fluorescent polarization that can then be measured using a fluorescence reader.

HDAC3 Assay Kit

55R-1377 100 assays
EUR 831.6
Description: Assay Kit for detection of HDAC3 in the research laboratory

HDAC8 Assay Kit

55R-1379 100 assays
EUR 826.8
Description: Assay Kit for detection of HDAC8 in the research laboratory

Lipase Assay Kit

abx098442-BeckmanR180ml1R220ml1 Beckman; R1:80ml×1 R2:20ml×1
EUR 830.4

Lipase Assay Kit

abx098442-Hitachi7170R140ml2R220ml1 Hitachi 7170; R1:40ml×2 R2:20ml×1
EUR 830.4

Lipase Assay Kit

abx098442-Hitachi7170R160ml1R215ml1 Hitachi 7170; R1:60ml×1 R2:15ml×1
EUR 679.2

Lipase Assay Kit

abx098442-Toshiba40R140ml1R210ml1 Toshiba 40; R1:40ml×1 R2:10ml×1
EUR 529.2

Lipase Assay Kit

abx098442-UniversalR120ml1R25ml1 Universal; R1:20ml×1 R2:5ml×1
EUR 379.2

Starch Assay Kit

abx097988-100Assays 100 Assays
EUR 529.2

PDE4B1 Assay Kit

79558 96 rxns.
EUR 425
Description: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. PDE4B1 isoform expression predominates in cortex, however lower expression of PDE4B1 has been observed in the cerebella of subjects with autism when compared with matched controls. The PDE4B1 Assay Kit is designed for identification of inhibitors of PDE4B1 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B1 to the binding agent._x000D_Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The PDE4B1 Assay Kit comes in a convenient 96-well format, with purified PDE4B1 enzyme, fluorescently labeled PDE4B1 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE4B3 Assay Kit

79574 96 rxns.
EUR 425
Description: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. PDE4B3 mRNA is readily expressed in both oligodendrocytes and neurons and it is the first cAMP-specific phosphodiesterase to be associated with hippocampal long-term potentiation (LTP), the most prominent cellular model for learning and memory formation. The PDE4B3 Assay Kit is designed for identification of inhibitors of PDE4B3 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B3 to the binding agent._x000D_Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The PDE4B3 Assay Kit comes in a convenient 96-well format, with purified PDE4B3 enzyme, fluorescently labeled PDE4B3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B3 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B3 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B3 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

NMNAT1 Assay Kit

79642 96 rxns.
EUR 510
Description: The NMNAT1 Inhibitor Screening Assay Kit is designed to measure NMNAT1 activity for screening and profiling applications. The NMNAT1 assay kit comes in a convenient 96-well format, with purified recombinant NMNAT1 enzyme, NMNAT1 assay buffer, NMN, and ATP sufficient for 96 enzyme reactions.

PDE4B2 Assay Kit

60343 96 rxns.
EUR 455
Description: The PDE4B2 Assay_x000D_Kit is designed for identification of inhibitors of PDE4B2 using fluorescence polarization. The_x000D_assay is based on the binding of a fluorescent nucleotide monophosphate generated by_x000D_PDE4B2 to the binding agent. The key to the PDE4B2 Assay Kit is the specific binding_x000D_agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B2_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing_x000D_PDE4B2 for 1 hour. Second, a binding agent is added to the reaction mix to produce a_x000D_change in fluorescent polarization that can then be measured using a fluorescence reader_x000D_equipped for the measurement of fluorescence polarization.

PDE4D2 Assay Kit

60345 96 rxns.
EUR 455
Description: The PDE4D2 Assay Kit is designed for identification of_x000D_inhibitors of PDE4D2 using fluorescence polarization. The assay is based on the binding of_x000D_a fluorescent nucleotide monophosphate generated by PDE4D2 to the binding agent. The key to the PDE4D2 Assay Kit is the specific binding_x000D_agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D2_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing_x000D_PDE4D2 for 1 hour. Second, a binding agent is added to the reaction mix to produce a_x000D_change in fluorescent polarization that can then be measured using a fluorescence reader_x000D_equipped for the measurement of fluorescence polarization._x000D_

PDE4D3 Assay Kit

60346 96 rxns.
EUR 425
Description: The PDE4D3 Assay Kit is designed for identification of_x000D_inhibitors of PDE4D3 using fluorescence polarization. The assay is based on the binding of_x000D_a fluorescent nucleotide monophosphate generated by PDE4D3 to the binding agent. The key to the PDE4D3 Assay Kit is the specific binding_x000D_agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D3_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing_x000D_PDE4D3 for 1 hour. Second, a binding agent is added to the reaction mix to produce a_x000D_change in fluorescent polarization that can then be measured using a fluorescence reader_x000D_equipped for the measurement of fluorescence polarization._x000D_

PDE5A1 Assay Kit

60350 96 rxns.
EUR 455
Description: The_x000D_PDE5A Assay Kit is designed for identification of PDE5A inhibitors using fluorescence_x000D_polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE5A to the binding agent. The key to the PDE5A Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE5A reactions. First, the fluorescently labeled cGMP is incubated with_x000D_PDE5A for 1 hour. Second, the binding agent is added to the reaction mix to produce a_x000D_change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader equipped for the measurement of fluorescence polarization.

PDE5A1 Assay Kit

60351 384 rxns.
EUR 800
Description: The PDE5A1 Assay Kit is designed for identification of PDE5A1 inhibitors_x000D_using fluorescence polarization. The assay is based on the binding of a fluorescent_x000D_nucleotide monophosphate generated by PDE5A to the binding agent. PDE5A catalyzes the_x000D_hydrolysis of the phosphodiester bond in dye-labeled cyclic guanosine monophosphate._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cyclic guanosine monophosphate. In_x000D_the polarization assay, dye molecules with absorption transition vectors parallel to the linearlypolarized_x000D_excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic_x000D_monophosphates will obtain random orientations and emit light with low polarization. Dyes_x000D_attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and_x000D_therefore will emit highly polarized light._x000D__x000D_The key to the PDE5A1 Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for_x000D_PDE5A1 reactions. First, the fluorescently labeled cGMP is incubated with a sample containing_x000D_PDE5A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change_x000D_in fluorescent polarization that can then be measured using a fluorescence reader.

PDE10A Assay Kit

60400 96 rxns.
EUR 405
Description: The PDE10A Assay Kit is designed for identification of PDE10A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A to the binding agent. The key to the PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE10A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then be_x000D_measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE11A Assay Kit

60411 96 rxns.
EUR 405
Description: The PDE11A Assay Kit is designed for identification of PDE11A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE11A to the_x000D_binding agent. The key to the PDE11A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE11A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE11A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader._x000D_

Cobalt Assay Kit

55R-1435 100 assays
EUR 745.2
Description: Assay Kit for detection of Cobalt in the research laboratory

Nickel Assay Kit

55R-1436 100 assays
EUR 745.2
Description: Assay Kit for detection of Nickel in the research laboratory

Malate Assay Kit

55R-1478 100 assays
EUR 807.6
Description: Assay Kit for detection of Malate in the research laboratory

Starch Assay Kit

55R-1482 100 assays
EUR 807.6
Description: Assay Kit for detection of Starch in the research laboratory

Lipase Assay Kit

55R-1506 100 assays
EUR 847.2
Description: Assay Kit for detection of Lipase in the research laboratory

Lipase Assay Kit

55R-1507 100 assays
EUR 888
Description: Assay Kit for detection of Lipase in the research laboratory

HSP90α Assay Kit

50293 96 rxns.
EUR 530
Description: The HSP90α Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

HSP90β Assay Kit

50294 96 rxns.
EUR 470
Description: The HSP90β Assay Kit is designed for identification of HSP90β inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90β. The key to the HSP90β Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90β reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90β enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

Tannin Assay Kit

abx298826-100Assays 100 Assays
EUR 585.6

Pepsin Assay Kit

abx298847-100Assays 100 Assays
EUR 510

Lipase Assay Kit

abx298849-100Assays 100 Assays
EUR 566.4

Kalium Assay Kit

abx298870-100Assays 100 Assays
EUR 472.8

Copper Assay Kit

abx298883-100Assays 100 Assays
EUR 510

Copper Assay Kit

Z5030024 250 assays
EUR 957

Lipase Assay Kit

Z5030051 100 assays
EUR 938

Calcium Assay Kit

abx098414-Hitachi7020R140ml2R240ml2 Hitachi 7020; R1: 40ml×2 R2: 40ml×2
EUR 247.2

Calcium Assay Kit

abx098414-Hitachi7060R190ml1R290ml1 Hitachi 7060; R1: 90ml×1 R2: 90ml×1
EUR 247.2

Calcium Assay Kit

abx098414-Toshiba40R140ml2R240ml2 Toshiba 40; R1: 40ml×2 R2: 40ml×2
EUR 247.2

Calcium Assay Kit

abx098414-UniversalR140ml2R240ml2 Universal; R1: 40ml×2 R2: 40ml×2
EUR 247.2

Glucose Assay Kit

abx098429-Hitachi7020R140ml4R240ml1 Hitachi 7020; R1: 40ml×4 R2: 40ml×1
EUR 266.4

Glucose Assay Kit

abx098429-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 266.4

Glucose Assay Kit

abx098429-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 266.4

NADPase Assay Kit

abx097983-100Assays 100 Assays
EUR 604.8

Glucose Assay Kit

abx090673-1Kit 1 Kit
EUR 284.4

PDE10A1 Assay Kit

60385 384 rxns.
EUR 800
Description: The PDE10A1 Assay Kit is designed for identification of PDE10A1 inhibitors_x000D_using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE10A1 to the binding agent. The key to the PDE10A1 Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for_x000D_PDE10A1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing_x000D_PDE10A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change_x000D_in fluorescent polarization that can then be measured using a fluorescence reader.

Calpain Assay Kit

55R-1342 100 assays
EUR 934.8
Description: Assay Kit for detection of Calpain in the research laboratory

We found that t / q-IgA have significantly higher capacity to reduce plaque size of IAVs of m-IgG and IgA, most likely caused by a decrease in the number of offspring produced virus particles from infected cells. Interestingly, HA-M2 colocalization was greatly reduced on the surface of infected cells with antibodies ch-rM2ss23. These results indicate that the polymeric IgA anti-M2 Limit beginner IAV more efficient than IgG and suggested the role of anti-M2 IgA cross-protective immunity IAVs.