Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

Background: polymerase chain reaction (PCR) which is used to detect viral pathogens because of the high sensitivity and specificity. However, the conventional PCR method can not determine the virus infectivity. Virus infectivity is conventionally checked by methods such as plaque test, although the test like this takes a few days. Long-Range quantitative reverse-transcription PCR (RT-qPCR) has previously been suggested for a rapid assessment of RNA virus infectivity which the loss of infectivity due to the fragmentation of the genome.


Methods: IAV is irradiated with ultraviolet rays of 253.7 nm (UV) to induce genomic strand breaks were confirmed by the full-length RT-PCR assay. The IAV is then subjected to the test plaque, conventional RT-qPCR and RT-qPCR long term to examine the relationship between infectious titer and number of copies. A simple linear regression analysis was conducted to test the correlation between the test results.


Results: A remote RT-qPCR assay was developed and validated for influenza A virus (IAV). Although only a few minutes of UV radiation required to completely disable IAV, genomic RNA remains detectable by conventional RT-qPCR and full-length RT-PCR for the following NS inactivation of the viral genome. A long-distance RT-qPCR assay was then designed using RT-priming at the 3 ‘termini of each segment and subsequent genomic qPCR of the 5’ region. UV-mediated inactivation IAV successfully analyzed by RT-qPCR assay distance especially when targeting the PA of the viral genome. It is also supported by a regression analysis that within RT-qPCR is highly correlated with plaque test (adjusted R2 = 0.931, P = 0.000066).


Conclusion
: This study shows that IAV infectivity can be predicted without testing infectivity. Rapid detection of pathogenic IAV has, therefore, been achieved with this sensing technology.

 Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay
Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

The one-step immunoassay without washing steps for the detection of influenza A virus using isfet

A one-step immunoassay for the detection of influenza A virus were developed using two different microbeads and bottle filter-inserted. Two types of beads with a diameter of 15 (capture beads) and 3 (detection beads) um prepared specifically detect the influenza virus A. Anti-influenza A virus antibody coated on both types of beads, while urease was immobilized only on the detection beads.

Samples of influenza A-positive can form a sandwich complex with the capture and detection of beads; This complex will not pass through the filter, which has a controlled pore size. As the beads used at a concentration detection limit, it will be prevented from crossing the filter; thus, the more it will react with the substrate urea and consequently increase the pH. Influenza A-negative samples will fail to form a sandwich complex in the presence of the capture and detection beads.

Thus, the detection beads will pass through the filter into urea buffer and increasing the pH. Changes in pH in the reaction of urease can be quantitatively measured by indicators such as phenol red or using ion-selective field-effect transistors (isfet). one-step immunoassay is used to detect influenza A viruses in real samples.

Cell Culture Grade Water, Cell Culture Tested, Sterile

CCM2081-1000 1000 mL
EUR 82.64

Gryphon Ecotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)

ABP-RVC-10002C 1 flask Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

LoxP Reporter Cell Lines: LoxP-RFP Human Fibroblast (1 x 106 cells, Culture)

ABP-RP-CRFPLOXC 1 flask Ask for price

Gryphon Amphotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)

ABP-RVC-10001C 1 flask Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CRFK CELL TUBE CULTURE

63-0600 each Ask for price

Water for Cell Culture

TBS5050 500ml
EUR 10

T175 Cell Culture Flask

P0220 40/case
EUR 215

T225 Cell Culture Flask

P0230 25/case
EUR 185

Cell Culture Grade Water

25-511 6 x 500 mL/Unit
EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water

25-511B 6 x 1000 mL
EUR 85.86
Description: Cell Culture Tested, Sterile

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

CELL CULTURE GRADE WATER

IB42010 1L
EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42011 6x1L
EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42012 12x1L
EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42020 2L
EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42021 6x2L
EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42030 10L
EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

100 mm Cell Culture Dish

P0300 300/case
EUR 250

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 162.61

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 8 rxn
EUR 499

Cellulase, Plant Culture Tested

PCT1518-100KU 1 unit
EUR 472.65
Description: Cellulase, Plant Culture Tested

Cellulase, Plant Culture Tested

PCT1518-25KU 1 unit
EUR 186.03
Description: Cellulase, Plant Culture Tested

Cellulase, Plant Culture Tested

PCT1518-50KU 1 unit
EUR 270.07
Description: Cellulase, Plant Culture Tested

Cellulase, Plant Culture Tested

PCT1518-5KU 1 unit
EUR 31.1
Description: Cellulase, Plant Culture Tested

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

6-Well Cell Culture Plates

25-105 100 Plates/Unit
EUR 119.75
Description: Flat Bottom Wells, TC Treated

HEPES. cell culture tested - kg

PM-B2093/1000 kg
EUR 146.69

6-well Cell Culture Plates

abx090688-100l 100 µl
EUR 125

6-well Cell Culture Plates

abx090688-1ml 1 ml Ask for price

6-well Cell Culture Plates

abx090688-200l 200 µl
EUR 306.25

Greiner culture flasks tissue culture treated - PK200

C6231-200EA PK200
EUR 260.55

12-Well Cell Culture Plates

25-106 100 Plates/Unit
EUR 123.27
Description: Flat Bottom Wells, TC Treated

24-Well Cell Culture Plates

25-107 100 Plates/Unit
EUR 125.99
Description: Flat Bottom Wells, TC Treated

48-Well Cell Culture Plates

25-108 100 Plates/Unit
EUR 138.07
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-109 100 plates
EUR 117.72
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-221 100 plates
EUR 139.75
Description: U-Bottom Wells, TC Treated

Cell Culture Inserts, 6-Well

25-281 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-282 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-283 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 8µm PC Membrane, Sterilized

Fish Serum (cell culture grade)

GWB-AE89C9 10 ml Ask for price

HEPES. cell culture tested - 100g

PM-B2093/100 100g
EUR 23.21

HEPES. cell culture tested - 250g

PM-B2093/250 250g
EUR 44

HEPES. cell culture tested - 500g

PM-B2093/500 500g
EUR 80.08

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4

Cell Culture Inserts, 12-Well

25-285 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 12-Well

25-286 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-287 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-288 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-289 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

ITS Cell Culture Supplement(100×)

ITSS-10201 10mL
EUR 59

Human CD34+ Cell Culture Medium

P771 8 rxn
EUR 299

3D Cell Culture Matrix BME Kit

K518-100 each
EUR 744

Cell Culture Freezing Medium (FBS)

cAP-22 50ml
EUR 108.9

Culture flask CELLine CL 350 - PK5

DD150102B PK5
EUR 2601.45

ExoStep Culture

ExoS-25-C9 25 test
EUR 423.5
Description: Exosome purification and FACS analysis kit of exosomes derived from cell culture

DMSO Bio-Max, Cell Culture Grade

40470005-1 50 mL
EUR 94.72

DMSO Bio-Max, Cell Culture Grade

40470005-2 100 mL
EUR 158.19

DMSO Bio-Max, Cell Culture Grade

40470005-3 25 mL
EUR 53.53

DMSO Bio-Max, Cell Culture Grade

40470005-4 250 mL
EUR 271.78

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 96

Mitochondria Isolation Kit (Cultured Cells)

K1138-10 10rxns
EUR 28
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-20 20rxns
EUR 44
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-30 30rxns
EUR 56
Description: Mitochondria Isolation Kit

500mL Water Cell Culture Grade - PK6

25-055-CV PK6
EUR 66.15

Gibco CHO Cell Culture Medium - EACH

220253 EACH
EUR 64.48

Cell Culture Supernatant Diluent Kit

DKC100 96 Tests
EUR 50

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

Multi-Layer Cell Culture Bioreactor

P0700 1
EUR 415

EZ-LINE Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile TC treated, 500 per case

SP31146 1CS, 500UNIT
EUR 208.27

Cell Culture Water Pyrogen free - 100ml

LM-T1707/100 100ml
EUR 2.59

Cell Culture Water Pyrogen free - 1000ml

LM-T1707/1000 1000ml
EUR 5.45

Cell Culture Water Pyrogen free - 500ml

LM-T1707/500 500ml
EUR 3.19

1L Water Cell Culture Grade WFI - PK6

25-055-CM PK6
EUR 95.85

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 10 mL
EUR 389

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 2 mL
EUR 189

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 10 mL
EUR 389

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 2 mL
EUR 189

CULTURE VESSEL, CULTUREJAR, PT-12

C185 100EA
EUR 48.82

Cell Culture Freezing Medium (NON-FBS)

cAP-22B 50ml
EUR 128.7

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 10 mL
EUR 389

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 2 mL
EUR 189

Ultrafiltration Water, cell culture level

PB180518-500mL 500 mL
EUR 15
Description: Supplements & Reagents

Ultrafiltration Water, cell culture level

PB180518 500mL
EUR 15
Description: Others

Stock Culture Agar

M182-500G 1 unit
EUR 44.15
Description: Stock Culture Agar

Algae Culture Agar

M343-500G 1 unit
EUR 45.2
Description: Algae Culture Agar

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 116.4

BD Biocoat 10cm cell culture dish - PK10

354469 PK10
EUR 126.9

Plates 96 wells flat bottom for cell culture Cellstar standard Greiner Bio-One - PK100

DD20034 PK100
EUR 256.5

Algae Culture Broth

M342-100G 1 unit
EUR 14.77
Description: Algae Culture Broth

Algae Culture Broth

M342-500G 1 unit
EUR 42.8
Description: Algae Culture Broth

TRAY, CULTURE VESSEL

T8077 1EA
EUR 133.43

Mouse Epithelial Cell culture Medium Kit

ABM-TM4285 500 ml Basal medium with the growth factor supplement Ask for price
Description: Epithelial Cell Medium is a complete medium designed for the culture of epithelial cells. It was tested and optimized with epithelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with epithelial cell growth supplement, antibiotics, and fetal bovine serum.Epithelial Cell Medium Supplement Kit includes:- 0.5 ML RI-Transferrin-Selenium (ITS)- 0.5 ML EGF- 5.0 ML L-Glutamine- 5.0 ML Antibiotic-Antimycotic Solution- 10.0 ML FBS*Before use, add Supplement Kit into 500ml basal medium.

Human B Cell Culture and Expansion Kit

BC1001-1 1 package
EUR 5200

3D Cell Culture Matrix Duo-Matrix Kit

K519-100 each
EUR 718.8

CLOSURE, CULTURE TUBE

C935 500EA
EUR 45.01

Cancer Antigen 50 (CA 50) GI cell culture

GWB-3F2336 50000 Unit Ask for price

Ampicillin sodium salt, cell culture grade

GA0283-100 100
EUR 189.8

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 265.2

Ampicillin sodium salt, cell culture grade

GA0283-25 25
EUR 71.1

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 122.4

Ampicillin sodium salt, cell culture grade

GA0283-5 5
EUR 23.8

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 64.8

Sodium Bicarbonate. cell culture tested - kg

PM-S2011/1 kg
EUR 10.4

Pluronic® F-127 *Cell culture tested *

20050 10 g
EUR 88
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

Pluronic® F-127 *Cell culture tested *

20050-10g 10 g
EUR 86
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 64.18

Sodium Bicarbonate. cell culture tested - 500g

PM-S2011/500 500g
EUR 6.71

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 64.7

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 65.02

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-010 100ml Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-050 500ml Ask for price

Multifuge X4 Pro cell culture package - EACH

75016067 EACH
EUR 18158.85

Carcinoembryonic Antigen (CEA) 95% Cell Culture

GWB-7FE58C 0.1 mg Ask for price

Carcinoembryonic Antigen (CEA) 95% Cell Culture

GWB-B045F9 1 mg Ask for price

Pluronic F-68, 10% Sol, Cell Culture tested

CA072-010 100ml
EUR 105.6

ExoStep Culture + Standard

ExoS-50-CST9 50
EUR 951.5
Description: Exosome purification and FACS analysis kit of exosomes derived from cell culture + Lyophilized Exosome from PC-3 cell line

Multifuge X4R Pro cell culture package - EACH

75016066 EACH
EUR 24460.65

Water, GlenCell™, suitable for cell culture

GK3940-1 1
EUR 55.4

Water, GlenCell™, suitable for cell culture

GK3940-1L 1 l
EUR 79.2

HEPES, 99.5%, GlenCell, suitable for cell culture

GM5581-1KG 1 kg
EUR 370.8

ExoFACS? Kit for Cell Culture Media Exosomes

K1236-20 each
EUR 973.2

Genomic DNA Extraction Mini KitBlood/Cultured Cells

FYG100-300P 300 Preps Ask for price

Genomic DNA Extraction Mini KitBlood/Cultured Cells

FYG101-100P 100 Preps Ask for price

Probenecid *Cell culture tested* *CAS 57-66-9*

20060-10x72mg 10x72 mg
EUR 86
Description: Probenecid is an inhibitor of organic-anion transporters located in cell membranes.

Pantothenate Culture Agar

M135-100G 1 unit
EUR 15.93
Description: Pantothenate Culture Agar

3D Cell Culture Matrix Alginate Hydrogel Kit

K517-100 each
EUR 666

Exosome Isolation kit (for cell culture media)

P100 10 rxn
EUR 199

Exosome Isolation kit (for cell culture media)

P100L 40 rxn
EUR 769

Exosome Isolation kit (for cell culture media)

P100S 2 rxn
EUR 69

culture flask 500ml - PK5

FLA4288 PK5
EUR 162

culture flask 1000ml - PK2

FLA4290 PK2
EUR 79.65

THP-1 Culture Medium

cAP-43 500ml
EUR 118.8

ExoStepTM Culture + Standard

ExoS-25-CST9 25 test
EUR 871.2

ECGF (Crude Extract, cell culture grade) + Heparin

300-090H 6mg
EUR 115.2

ExKine™ Mitochondrion Extraction Kit (Cultured Cells)

KTP4003-200T 200 T
EUR 149
Description: Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables isolation of of intact mitochondria from cultured mammalian cells.

ExKine™ Mitochondrion Extraction Kit (Cultured Cells)

KTP4003-50T 50 T
EUR 49
Description: Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables isolation of of intact mitochondria from cultured mammalian cells.

CULTURE TUBE, FLIP-CAP

C1898 250EA
EUR 31.02

culture flask 250ml - PK10

FLA4286 PK10
EUR 326.7

culture flask 2000ml - EACH

FLA4292 EACH
EUR 54

Folic Acid Culture Agar

M134-100G 1 unit
EUR 14.77
Description: Folic Acid Culture Agar

CORNING® ENDOTHELIAL CELL CULTURE MEDIUM, KIT

355054 1/pk
EUR 478.8
Description: Advanced Cells - DL; Adv Cell Misc Custom - DL

L-Alanine, GlenCell, suitable for cell culture

GM0361-1KG 1 kg
EUR 303.6

CULTURE VESSEL, PTL-100

C2100 100EA
EUR 60.72

CULTURE MEDIUM BOTTLE - 4L

ACCFL0008 POLYCARBONATE
EUR 288.29

HEPES, 99.5%, GlenCell™, suitable for cell culture

GM5581-1 1
EUR 276.9

Receiver operating characteristics (ROC) plot analysis shows the value of area under the curve (AUC) of 0.931; the sensitivity and specificity of the test was 80% and 90%, respectively, with a cutoff value of 0.9986. These results indicate that one-step immunoassay can increase the sensitivity of detection of influenza A viruses in real samples.